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clonealign

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clonealign assigns single-cell RNA-seq expression to cancer clones by probabilistically mapping RNA-seq to clone-specific copy number profiles using reparametrization gradient variational inference. This is particularly useful when clones have been inferred using ultra-shallow single-cell DNA-seq meaning SNV analysis is not possible.

See the website for more details as well as the introductory vignette.

Getting started

Installation

clonealign is built using Google’s Tensorflow so requires installation of the R package tensorflow:

install.packages("tensorflow")
tensorflow::install_tensorflow(extra_packages ="tensorflow-probability", version="1.12.0")

Note that clonealign uses the Tensorflow probability library, requiring Tensorflow version >= 1.12.0, which can be installed using the above.

clonealign can then be installed from github:

install.packages("devtools") # If not already installed
install_github("kieranrcampbell/clonealign")

Usage

clonealign accepts either a cell-by-gene matrix of raw counts or a SingleCellExperiment with a counts assay as gene expression input. It also requires a gene-by-clone matrix or data.frame corresponding to the copy number of each gene in each clone. The cells are then assigned to their clones by calling

cal <- clonealign(gene_expression_data, # matrix or SingleCellExperiment
                  copy_number_data)     # matrix or data.frame
print(cal)
A clonealign_fit for 200 cells, 100 genes, and 3 clones
To access clone assignments, call x$clone
To access ML parameter estimates, call x$ml_params
print(head(cal$clone))
[1] "B" "C" "C" "B" "C" "B"

Paper

https://www.biorxiv.org/content/early/2018/06/11/344309

Authors

Kieran R Campbell, University of British Columbia